CF633 donkey anti mouse

Properties

Biological source: Rabbit

Quality level: 200

Antibody form: IgG fraction of antiserum

Antibody product type: secondary antibodies

Clone: polyclonal

Form: buffered aqueous solution

Application(s)

  • Direct ELISA: 1: 40,000 using 5 μg / ml mouse IgG for coating and OPD substrate
  • immunohistochemistry (paraffin-embedded sections, formalin-fixed): 1: 200
  • Western blot (chemiluminescent): 1: 80,000-160,000

Conjugate: peroxidase conjugate

Sent in: dry ice

Storage temperature: −20 ° C

Related categories: Secondary antibodies

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein and an important class of immunoglobulin. Immunoglobulin G (IgG) is involved in type II and type III hypersensitivity. The mouse consists of five classes of immunoglobulins: IgM, IgG, IgA, IgD, and IgE. Mouse IgG is divided into five classes: IgG1, IgG2a, IgG2b, and IgG3. IgG aids in opsonization, complement fixation, and antibody-dependent cell-mediated cytotoxicity.

The subtype of IgG antibodies is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in the blood and extracellular fluids. The IgG antibody is a glycoprotein, composed of two polypeptide chains (light and heavy), each of which has variable and constant domains.

The variable region of the IgG antibody is antigen-specific and highly conserved. The anti-mouse IgG (whole molecule) -Peroxidase antibody is specific for all subclasses of mouse IgG. IgG is purified from mouse serum by fractionation and ion-exchange chromatography. The purified IgG is then conjugated to peroxidase by protein crosslinking with 0.2% glutaraldehyde.

Specificity

The anti-mouse IgG (whole molecule) -Peroxidase antibody is specific for all subclasses of mouse IgG.

Immunogen

Purified mouse IgG

Request

Epidermal protein extracts were generated from human breast surgery samples and Western blotted using HRP-conjugated rabbit anti-mouse IgG as secondary to a 1: 80,000 dilution in TBSt / 5% milk.

Anti-mouse IgG (whole molecule): The peroxidase antibody can be used in direct ELISA at a working antibody dilution of 1: 40,000. For immunoblotting, a dilution of 1: 80,000 to 1: 160,000 can be used. A working antibody dilution of 1: 200 is recommended for immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil sections of the human appendix. Mouse IgG-peroxidase antibody at a 1:20 dilution was used for immunohistochemistry of mouse muscle tissue sections; A dilution of 1: 500 was used for the southwest transfer in the same study.

For immunoblotting of human skeletal muscle tissue extracts, a 1: 5000 dilution was used. Anti-mouse IgG (whole molecule): The peroxidase antibody has been used in immunohistochemical, immunohistochemical, and protein pin matrix assays.

Biochemical / Physiological Actions

The IgG antibody provides protection against infections caused by bacteria, fungi, and viruses. Maternal IgG is transferred to the fetus through the placenta, which is vital for the newborn’s immune defense against infection. The IgG antibody has a function similar to that of the IgM antibody in activating the complement system.

Physical form

Solution in 0.01 M phosphate-buffered saline, pH 7.4, containing 0.05% MIT

Preparation note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Storage and stability

Store at -20 ° C for an extended period. For continuous use, the product can be stored at 2-8 ° C for up to one month. For long-term storage, the solution can be frozen in working aliquots at -20 ° C. Repeated freezing and thawing, or storing in “frost-free” freezers, is not recommended. If slight cloudiness occurs after prolonged storage, rinse the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product (s), our products are designed for research use only and are not to be used for any other purpose, including but not limited to commercial uses. unauthorized, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption or application to humans or animals.

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