Genome-wide characterization, evolution, and expression profiling

trancheproject

trancheproject

Genome-wide characterization, evolution, and expression profiling of FBA gene family in response to delicate therapies and abiotic stress in Nicotiana tabacum

Fructose 1,6-bisphosphate aldolase (FBA) as a key enzyme play important roles in glycolysis, gluconeogenesis and Calvin cycle processes in crops. Nonetheless, restricted data is known referring to FBA genes in Nicotiana tabacum. On this analysis, 16 FBAs had been acknowledged and characterised in Nicotiana tabacum. Phylogenetic analysis revealed that these genes could be categorized as kind I (NtFBA1-10 located in chloroplast) and type II (NtFBA11-16 located in cytoplasm) subfamilies.

In step with the conserved motifs and gene development analysis, NtFBA protein sequences had the extraordinarily homologous to FBAs in several species. Most members of the NtFBA gene family responded positively to NaHCO3 stress, notably the expression of NtFBA13/14 elevated by 642%. In addition to, the expression outcomes of NtFBAs beneath 5 abiotic stress (delicate, NaCl, NaHCO3, drought, and chilly) conditions had been confirmed that NtFBA13/14 had been extraordinarily up-regulated. qRT-PCR outcomes confirmed that quite a lot of the NtFBAs expressed bigger in leaves.

NtFBA7/8 and NtFBA13/14 have important significance in photosynthesis and abiotic stress, respectively. This analysis affords a basis foundation for extra elucidating the carry out of NtFBAs and the N. tabacum mechanism of resistance beneath abiotic stress.

trancheproject
trancheproject

Novel Coronavirus COVID-19 (2019-nCoV) Real Time Multiplex RT-PCR Kit (Detection for 3 Genes )

RR-0479-02 25 tests/kit
EUR 1347
  • For use with PE5700, MJ-Opticon & other single color systems, ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX 96, Life 96, Slan 96, iCycl
  • Show more
Description: Novel Coronavirus (2019-nCoV) Real Time Multiplex RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. It detects N gene, E gene and RdRp gene of 2019-nCoV. RR-0479-02 has been also approverd by CFDA for emergency use and is WHO standard.

hsa-mir-521 Real-time RT-PCR Detection Kit

20-abx097600
  • EUR 551.00
  • EUR 787.00
  • EUR 398.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

MDV Real-Time PCR Kit

PD65-06 96t
EUR 1553.6
Description: Please check the datasheet of MDV Real-Time PCR Kit before using the test.

ALV Real-Time PCR Kit

PD65-15 96t
EUR 1724.4
Description: Please check the datasheet of ALV Real-Time PCR Kit before using the test.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit

RR-0478-02 25 tests/kit
EUR 991
  • For use with PE5700, MJ-Opticon & other single color systems, ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX 96, Life 96, Slan 96, iCycl
  • Show more
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.

Canine coronavirus PCR kit

PCR-V110-48R 50T
EUR 524.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Canine coronavirus

Canine coronavirus PCR kit

PCR-V110-96R 100T
EUR 669.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Canine coronavirus

Feline coronavirus PCR kit

PCR-V177-48R 50T
EUR 524.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Feline coronavirus

Feline coronavirus PCR kit

PCR-V177-96R 100T
EUR 669.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Feline coronavirus

Bovine coronavirus PCR kit

PCR-V220-48R 50T
EUR 524.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Bovine coronavirus

Bovine coronavirus PCR kit

PCR-V220-96R 100T
EUR 669.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Bovine coronavirus

rat coronavirus PCR kit

PCR-V383-48R 50T
EUR 524.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of rat coronavirus

rat coronavirus PCR kit

PCR-V383-96R 100T
EUR 669.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of rat coronavirus

Equine Coronavirus PCR kit

PCR-V587-48R 50T
EUR 524.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Equine Coronavirus

Equine Coronavirus PCR kit

PCR-V587-96R 100T
EUR 669.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Equine Coronavirus

dsGreen for Real-Time PCR, 100×, 100 uL

11010 100 uL
EUR 59

dsGreen for Real-Time PCR, 100×, 2 mL

61010 2 mL
EUR 363

dsGreen for Real-Time PCR, 100×, 1.5 mL

51010 1.5 mL
EUR 331

dsGreen for Real-Time PCR, 100×, 1 mL

41010 1 mL
EUR 249

dsGreen for Real-Time PCR, 100×, 10 mL

81010 10 mL
EUR 869

dsGreen for Real-Time PCR, 100×, 5 mL

71010 5 mL
EUR 625

dsGreen for Real-Time PCR, 100×, 50 mL

91010 50 mL
EUR 3169

hsa-mir-521 Real-Time RT-PCR Detection and U6 Calibration Kit

20-abx097601
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

Human Coronavirus alpha PCR kit

PCR-H717-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Human Coronavirus alpha

Human Coronavirus alpha PCR kit

PCR-H717-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Human Coronavirus alpha

Human Coronavirus beta PCR kit

PCR-H718-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Human Coronavirus beta

Human Coronavirus beta PCR kit

PCR-H718-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Human Coronavirus beta

Swine delta coronavirus PCR kit

PCR-V134-48R 50T
EUR 524.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Swine delta coronavirus

Swine delta coronavirus PCR kit

PCR-V134-96R 100T
EUR 669.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Swine delta coronavirus

Transmissible Gastroenteritis Coronavirus PCR kit

PCR-V140-48R 50T
EUR 524.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Transmissible Gastroenteritis Coronavirus

Transmissible Gastroenteritis Coronavirus PCR kit

PCR-V140-96R 100T
EUR 669.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Transmissible Gastroenteritis Coronavirus

Ferret enteric coronavirus PCR kit

PCR-V377-48R 50T
EUR 524.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Ferret enteric coronavirus

Ferret enteric coronavirus PCR kit

PCR-V377-96R 100T
EUR 669.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Ferret enteric coronavirus

Ferret systemic coronavirus PCR kit

PCR-V378-48R 50T
EUR 524.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Ferret systemic coronavirus

Ferret systemic coronavirus PCR kit

PCR-V378-96R 100T
EUR 669.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Ferret systemic coronavirus

Coronavirus (SARS-CoV-2) PCR Detection Kit

K1460 100 Rxns
EUR 570
  • The kit includes: Non-Template Negative Control (NTC), COVID-19 Positive control (PTC), PCR Primer/ Probe set, 2X qPCR Master Mix, Rehydration Buffer and Reverse Transcription Mix
Description: Kit for detection of SARS-CoV-2 in respiratory specimens using Real-Time (RT-PCR).

Coronavirus (SARS-CoV-2) PCR Detection Kit

K1460-100 100 Rxns Ask for price

Coronavirus NL63, 229E, OC43, HKU1 PCR kit

PCR-H715-48R 50T
EUR 524.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Coronavirus NL63, 229E, OC43, HKU1

Coronavirus NL63, 229E, OC43, HKU1 PCR kit

PCR-H715-96R 100T
EUR 669.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Coronavirus NL63, 229E, OC43, HKU1

hsa-let-7a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097603
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-let-7b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097604
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-let-7c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097605
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-let-7d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097606
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-let-7e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097607
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097608
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-let-7g Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097609
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-let-7i Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097610
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-let-7f Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097611
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-1 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097612
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-7 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097613
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-9 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097614
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-9* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097615
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-10a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097616
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-10b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097617
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-10a* Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097618
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-15a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097619
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-15b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097620
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-16 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097622
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-18a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097625
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-18b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097626
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-19a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097627
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-19b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097628
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-20a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097629
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-20b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097630
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-21 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097631
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-22 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097632
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-23a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097633
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-23b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097634
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-25 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097636
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-26a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097637
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-26b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097638
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-27a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097639
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-27b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097640
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-29a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097642
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-29b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097643
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-29c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097644
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-30a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097645
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-30b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097647
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-30c Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097648
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-30d Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097650
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-30e Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097651
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-31 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097652
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-32 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097653
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-33a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097654
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-34a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097657
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-34b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097659
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-92a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097661
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-92b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097662
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-93 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097663
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-95 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097664
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-96 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097665
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-98 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097666
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-99a Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097667
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-99b Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097669
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-100 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097671
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-101 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097673
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-103 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097675
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

hsa-mir-105 Real-Time RT-PCR Detection and cel-mir-39-3p Calibration Kit

20-abx097676
  • EUR 732.00
  • EUR 1038.00
  • EUR 523.00
  • 100 rxns
  • 200 rxns
  • 50 rxns
  • Shipped within 3 weeks.

Computational Analysis and Phylogenetic Clustering of SARS-CoV-2 Genomes

COVID-19, the sickness introduced on by the novel SARS-CoV-2 coronavirus, originated as an isolated outbreak inside the Hubei province of China nonetheless shortly created a worldwide pandemic and is now a critical menace to healthcare applications worldwide. Following the quick human-to-human transmission of the an an infection, institutes all around the world have made efforts to generate genome sequence information for the virus.

With a whole bunch of genome sequences for SARS-CoV-2 now accessible inside the public space, it is attainable to research the sequences and purchase a deeper understanding of the sickness, its origin, and its epidemiology. Phylogenetic analysis is a in all probability extremely efficient software program for monitoring the transmission pattern of the virus with a view to aiding identification of potential interventions.

In the direction of this goal, now now we have created an entire protocol for the analysis and phylogenetic clustering of SARS-CoV-2 genomes using Nextstrain, a powerful open-source software program for the real-time interactive visualization of genome sequencing information. Approaches to focus the phylogenetic clustering analysis on a selected space of curiosity are detailed on this protocol.

Genome-Huge Affiliation Analysis of Kernel Traits in Aegilops tauschii

Aegilops tauschii is the diploid progenitor of the D subgenome of hexaploid wheat (Triticum aestivum L.). Proper right here, the phenotypic information of kernel measurement (KL), kernel width (KW), kernel amount (KV), kernel flooring area (KSA), kernel width to measurement ratio (KWL), and hundred-kernel weight (HKW) for 223 A. tauschii accessions had been gathered all through three regular years. Based totally on inhabitants development analysis, 223 A. tauschii had been divided into two subpopulations, notably T-group (primarily included A. tauschii ssp. tauschii accessions) and S-group (primarily included A. tauschii ssp. strangulata). Classifications based totally on cluster analysis had been extraordinarily consistent with the inhabitants development outcomes. Within the meantime, the extent of linkage disequilibrium decay distance (r 2 = 0.5) was about 110 kb and 290 kb for T-group and S-group, respectively.

Furthermore, a genome-wide affiliation analysis was carried out on these kernel traits using 6,723 single nucleotide polymorphism (SNP) markers. Sixty-six necessary markers, distributed on all seven chromosomes, had been acknowledged using a blended linear model explaining 4.82-13.36% of the phenotypic variations. Amongst them, 15, 28, 22, 14, 21, and 13 SNPs had been acknowledged for KL, KW, KV, KSA, KWL, and HKW, respectively. Moreover, six candidate genes which is able to administration kernel traits had been acknowledged (AET2Gv20774800, AET4Gv20799000, AET5Gv20005900, AET5Gv20084100, AET7Gv20644900, and AET5Gv21111700). The change of useful genes from A. tauschii to wheat using marker-assisted selection will broaden the wheat D subgenome improve the effectivity of breeding.

Genome-Huge Analysis of Coding and Non-coding RNA Reveals a Conserved miR164-NAC-mRNA Regulatory Pathway for Sickness Safety in Populus

MicroRNAs (miRNAs) contribute to plant safety responses by rising the final genetic selection; nonetheless, their origins and helpful significance in plant safety keep unclear. Proper right here, we employed Illumina sequencing experience to guage how miRNA and messenger RNA (mRNA) populations fluctuate inside the Chinese language language white poplar (Populus tomentosa) all through a leaf black spot fungus (Marssonina brunnea) an an infection.

We sampled RNAs from infective leaves at conidia germinated stage [12 h post-inoculation (hpi)], infective vesicles stage (24 hpi), and intercellular infective hyphae stage (48 hpi), three necessary ranges associated to plant colonization and biotrophic progress in M. brunnea fungi. In complete, 8,938 conserved miRNA-target gene pairs and three,901 Populus-specific miRNA-target gene pairs had been detected. The result confirmed that Populus-specific miRNAs (66%) had been further involved inside the regulation of the sickness resistance genes. In opposition to this, conserved miRNAs (>80%) objective further whole-genome duplication (WGD)-derived transcription elements (TFs).

Among the many many 1,023 WGD-derived TF pairs, 44.9% TF pairs had only one paralog being centered by a miRNA that could be on account of each purchase or lack of a miRNA binding web site after the WGD. A conserved hierarchical regulatory group combining promoter analyses and hierarchical clustering technique uncovered a miR164-NAM, ATAF, and CUC (NAC) transcription factor-mRNA regulatory module that has potential in Marssonina safety responses. Furthermore, analyses of the areas of miRNA precursor sequences reveal that pseudogenes and transposon contributed a certain proportion (∼30%) of the miRNA origin. Collectively, these observations current evolutionary insights into the origin and potential roles of miRNAs in plant safety and helpful innovation.

Genome-Huge Affiliation Analysis Reveal Susceptibility Loci for Noninfectious Claw Lesions in Holstein Dairy Cattle

Sole ulcers (SUs) and white line sickness (WLD) are two frequent noninfectious claw lesions (NICL) that come up on account of a compromised horn manufacturing and are frequent causes of lameness in dairy cattle, imposing welfare and profitability points. Low to common heritability estimates of SU and WLD susceptibility level out that genetic selection would possibly reduce their prevalence.

To find out the susceptibility loci for SU, WLD, SU and/or WLD, and any form of noninfectious claw lesion, genome-wide affiliation analysis (GWAS) had been carried out using generalized linear blended model (GLMM) regression, chunk-based affiliation testing (CBAT), and a random forest (RF) technique. Cows from 5 industrial dairies in California had been categorised as controls having no lameness data and ≥6 years earlier (n = 102) or circumstances having SU (n = 152), WLD (n = 117), SU and/or WLD (SU + WLD, n = 198), or any form of noninfectious claw lesion (n = 217).

The very best single nucleotide polymorphisms (SNPs) had been outlined as these passing the Bonferroni-corrected suggestive and significance thresholds inside the GLMM analysis or those that a validated RF model considered important. Outcomes of the best SNPs had been quantified using Bayesian estimation. Linkage disequilibrium (LD) blocks outlined by the best SNPs had been explored for candidate genes and beforehand acknowledged, functionally associated quantitative trait loci.

The GLMM and CBAT approaches revealed the equivalent areas of affiliation on BTA8 for SU and BTA13 frequent to WLD, SU + WLD, and NICL. These SNPs had outcomes significantly utterly completely different from zero, and the LD blocks they outlined outlined a necessary amount of phenotypic variance for each dataset (6.1-8.1%, p < 0.05), indicating the small nonetheless notable contribution of these areas to susceptibility. These areas contained candidate genes involved in wound therapeutic, pores and pores and skin lesions, bone progress and mineralization, adipose tissue, and keratinization.

The LD block outlined by primarily crucial SNP on BTA8 for SU included a SNP beforehand associated to SU. The RF fashions had been overfitted, indicating that the SNP outcomes had been very small, thereby stopping vital interpretation of SNPs and any downstream analyses. These findings urged that variants associated to quite a few physiological applications may contribute to susceptibility for NICL, demonstrating the complexity of genetic predisposition.

 

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