Properties

Quality level: 100

Biological source: mouse

Antibody form: purified immunoglobulin

Antibody product type: primary antibodies

Clone

• 13.1, monoclonal
• 7.1, monoclonal

Test: > 90% (HPLC)

Form: lyophilized

Packaging: 200 μg package

mfr. Not.: Roche

Isotype: IgG1κ

Related categories: Roche® Life Science Products

General description

Green fluorescent protein (GFP) is a spontaneously isolated 27 kDa fluorescent protein originally isolated from the jellyfish Aequorea victoria. Molecular cloning of the GFP gene and its subsequent expression in heterologous systems has established GFP as a valuable reporter molecule for in vivo visualization of gene expression events in a wide variety of cell types and organisms.

Since GFP does not require additional substrates or cofactors, the green fluorescence of GFP can be easily detected using blue or UV light after expression in prokaryotic or eukaryotic cells. In addition, several mutant forms of GFP have been reported with unique spectral properties (eg, enhanced fluorescence signal and changes in excitation and emission spectra). A mix of two high-affinity monoclonal antibodies selected for their performance in the detection of GFP and GFP fusion proteins.

Specificity

Subtype: Both clones are mouse IgG1κ

Request

Monoclonal antibody for the detection of mutant and wild-type forms of GFP or GFP fusions by:

• Immunoprecipitation
• Western spots
• Immunostaining

Features and Benefits

• A mix of two monoclonal antibodies, supplied as a white lyophilized containing 200 μg of total anti-GFP IgG.
• Anti-GFP is a mixture of two clones (7.1 and 13.1).

Quality

Anti-GFP is tested for functionality and purity against a reference standard to confirm the quality of each new reagent preparation.

Purity: Both mouse anti-GFP monoclonal antibodies (Clones 7.1 and 13.1) are> 95% pure as determined by ion-exchange HPLC analysis and SDS-PAGE.

Preparation note

Working concentration: The working concentration of the antibody depends on the application and the substrate.

The following concentrations should be taken as a guideline:

• Western blot: 1: 1000 dilution
• Immunoprecipitation: 2 to 10 μg
• Storage conditions (working solution): -15 to -25 ° C

Reconstitution

• Add 500 μl of double distilled water to a final concentration of 0.4 mg/ml.
• Rehydrate on ice for 30 minutes.

Other notes

For life science research only. It should not be used in diagnostic procedures.

Legal information

This product is sold under license from Columbia University. The rights to use this product are limited to research use only. No other rights are transferred. Inquiries regarding the availability of a license for broader rights or the use of this product for commercial purposes should be directed to Columbia Innovation Enterprise, Columbia University, Engineering Terrace – Suite 363, New York, New York 10027.